3RLS
Crystal structure of yeast AF-9 homolog protein Yaf9
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-23 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.643, 44.267, 62.158 |
| Unit cell angles | 71.78, 89.86, 83.31 |
Refinement procedure
| Resolution | 59.000 - 1.700 |
| R-factor | 0.16001 |
| Rwork | 0.158 |
| R-free | 0.20193 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fk3 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.510 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 33124 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 26% PEG 4000, 0.1M Tris-Cl, 0.1M Guanidine Hydrochloride, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
