3RLO
Structural Basis of Cytosolic DNA Recognition by Innate Receptors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2010-11-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0333 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 128.562, 128.562, 67.743 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.800 |
| R-factor | 0.1691 |
| Rwork | 0.168 |
| R-free | 0.19210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.207 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_723)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.083 | 0.489 |
| Number of reflections | 38686 | |
| <I/σ(I)> | 13.54 | 1.73 |
| Completeness [%] | 97.4 | 79.1 |
| Redundancy | 3.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 20% PEG1500, 10 mM MgCl2, 0.1 M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
