3RL8
Crystal structure of hDLG1-PDZ2 complexed with APC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-30 |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.806, 52.502, 87.426 |
| Unit cell angles | 90.00, 102.42, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.2062 |
| Rwork | 0.204 |
| R-free | 0.24490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zok |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.306 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 85.380 | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 4.740 | 2.200 |
| Rmerge | 0.088 | 0.074 | 0.216 |
| Number of reflections | 30730 | ||
| <I/σ(I)> | 17.6 | ||
| Completeness [%] | 99.8 | 98.4 | 99.9 |
| Redundancy | 6 | 5.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 287 | 0.8M Na2HPO4, 0.9M KH2PO4, pH 7.5, vapor diffusion, hanging drop, temperature 287K |
