3RKG
Structural and Functional Characterization of the Yeast Mg2+ Channel Mrs2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-09 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.880, 61.880, 85.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.177 - 1.280 |
| R-factor | 0.1704 |
| Rwork | 0.169 |
| R-free | 0.20390 |
| Structure solution method | SSAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.881 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.119 | 1.350 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.069 | 1.172 |
| Number of reflections | 75437 | |
| <I/σ(I)> | 19.9 | 1.83 |
| Completeness [%] | 92.1 | 79 |
| Redundancy | 12 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.3 | 295 | 22% v/v ethylene glycol, 56 mM Na/K phosphate, pH 6.3, VAPOR DIFFUSION, temperature 295K |
