3RKF

Crystal structure of guanine riboswitch C61U/G37A double mutant bound to thio-guanine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.02
Spacegroup name	P 32
Unit cell lengths	52.300, 52.300, 263.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.638 - 2.500
R-factor	0.2084
Rwork	0.207
R-free	0.22640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u8d
RMSD bond length	0.002
RMSD bond angle	0.492
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.640	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	26059
Completeness [%]	93.5	82.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	5mM K+ - HEPES, 12mM [Co(NH3)6]3+, 25% polyethylene glycol 4000, 450mM ammonium acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K