3RJY

Crystal Structure of Hyperthermophilic Endo-beta-1,4-glucanase in complex with substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-03
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 41 21 2
Unit cell lengths	47.300, 47.300, 271.387
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.570 - 2.200
R-factor	0.19039
Rwork	0.187
R-free	0.25276
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rjx
RMSD bond length	0.011
RMSD bond angle	1.298
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Number of reflections	16724
Completeness [%]	98.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	291	1.2M NaH2PO4/KH2PO4 (1:1), 0.1M sodium citrate pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	5.6	291	1.2M NaH2PO4/KH2PO4 (1:1), 0.1M sodium citrate pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K
1	VAPOR DIFFUSION, HANGING DROP	5.6	291	1.2M NaH2PO4/KH2PO4 (1:1), 0.1M sodium citrate pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K