3RJQ

Crystal structure of anti-HIV llama VHH antibody A12 in complex with C186 gp120

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Detector technology	CCD
Collection date	2010-04-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	66.600, 66.600, 266.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.473 - 2.601
R-factor	0.2572
Rwork	0.255
R-free	0.29880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.883
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.601
Number of reflections	13406
Completeness [%]	68.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	50 mM of KH2PO4, 24.2% PEG 8000, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K