3RJL
Crystal structure of 1-pyrroline-5-carboxylate dehydrogenase from Bacillus licheniformis (Target NYSGRC-000337)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-17 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.267, 116.114, 181.768 |
| Unit cell angles | 90.00, 90.15, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.22433 |
| Rwork | 0.222 |
| R-free | 0.29218 |
| Structure solution method | MR |
| Starting model (for MR) | 3qan |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.196 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 217382 | |
| <I/σ(I)> | 4.9 | 1 |
| Completeness [%] | 87.7 | 88.7 |
| Redundancy | 2.9 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 0.1M SODIUM ACETATE, PH 4.6, 200MM CADMIUM CHLORIDE, 30% PEG400, 10% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |
