3RJ8
Crystal structure of carbohydrate oxidase from Microdochium nivale
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-11-24 |
| Detector | MAR555 FLAT PANEL |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.710, 56.960, 87.050 |
| Unit cell angles | 90.00, 95.85, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.400 |
| R-factor | 0.156 |
| Rwork | 0.155 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zr6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.320 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0060) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.098 | 0.362 |
| Number of reflections | 24502 | |
| <I/σ(I)> | 11.7 | 3.42 |
| Completeness [%] | 95.5 | 95.2 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.01 M zinc sulfate, 0.1 M MES, 12% PEG550 MME, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
