3RJ0
Plant steroid receptor BRI1 ectodomain in complex with brassinolide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-12-22 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 175.170, 67.236, 119.246 |
| Unit cell angles | 90.00, 121.42, 90.00 |
Refinement procedure
| Resolution | 24.637 - 2.541 |
| R-factor | 0.1867 |
| Rwork | 0.184 |
| R-free | 0.24080 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.053 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.640 | 2.630 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Number of reflections | 38900 | |
| <I/σ(I)> | 14.9 | 2.3 |
| Completeness [%] | 98.6 | 93.8 |
| Redundancy | 4.1 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 14% PEG4000, 0.2 M ammonium sulfate, 0.1 M citric acid, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
