3RIR

Crystal Strucrture of Biotin Protein Ligase from S. aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 42 21 2
Unit cell lengths	93.564, 93.564, 130.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.925 - 2.600
R-factor	0.2091
Rwork	0.205
R-free	0.28520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wq7
RMSD bond length	0.021
RMSD bond angle	1.997
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.2_432))

Data quality characteristics

	Overall
Low resolution limit [Å]	35.000
High resolution limit [Å]	2.600
Rmerge	0.113
Number of reflections	18098
<I/σ(I)>	7.1
Completeness [%]	96.1
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1