3RIO

Crystal structure of GlcT CAT-PRDI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-11-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.999
Spacegroup name	P 21 21 21
Unit cell lengths	35.755, 47.597, 126.779
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.390 - 1.990
R-factor	0.2118
Rwork	0.210
R-free	0.24650
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.120
Data reduction software	XDS
Data scaling software	SADABS
Phasing software	SHELXD
Refinement software	BUSTER (2.8.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.390	2.100
High resolution limit [Å]	1.990	1.990
Number of reflections	15190
Completeness [%]	98.2	97.1
Redundancy		3.15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	Na formate, PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP		298	Na formate, PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K