3RIG
Sirt5 is an NAD-dependent protein lysine demalonylase and desuccinylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.917 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.538, 67.876, 156.749 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.221 |
| Rwork | 0.219 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b4y |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.856 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 5.290 | 1.950 |
| Rmerge | 0.090 | 0.071 | 0.346 |
| Number of reflections | 41492 | ||
| <I/σ(I)> | 9.6 | ||
| Completeness [%] | 99.9 | 97.9 | 99.7 |
| Redundancy | 6.8 | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | 20% PEG 8000, 0.1M CHES, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
