3RIC
Crystal Structure of D48V||A47D mutant of Human Glycolipid Transfer Protein complexed with 3-O-sulfo-galactosylceramide containing nervonoyl acyl chain (24:1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-03-22 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.871, 48.128, 69.086 |
| Unit cell angles | 90.00, 123.82, 90.00 |
Refinement procedure
| Resolution | 14.980 - 2.100 |
| R-factor | 0.18378 |
| Rwork | 0.182 |
| R-free | 0.21881 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.658 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.050 | 0.550 |
| Number of reflections | 11907 | |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 96.0 | 93.7 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.1 | 273 | 15-20% PEG 3350, pH 5.1, VAPOR DIFFUSION, HANGING DROP, temperature 273K |
