3RI8
Xylanase C from Aspergillus kawachii D37N mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 293 |
Detector technology | DIFFRACTOMETER |
Collection date | 1998-12-03 |
Detector | WEISSENBERG |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 62.064, 62.064, 113.382 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.700 - 2.000 |
R-factor | 0.17084 |
Rwork | 0.168 |
R-free | 0.22800 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1bk1 |
RMSD bond length | 0.022 |
RMSD bond angle | 2.122 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 14918 | |
<I/σ(I)> | 32.6 | 2.8 |
Completeness [%] | 95.2 | 86.3 |
Redundancy | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.5M sodium sulfate, 0.1M HEPES-NaOH, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |