3RI6
A Novel Mechanism of Sulfur Transfer Catalyzed by O-Acetylhomoserine Sulfhydrylase in Methionine Biosynthetic Pathway of Wolinella succinogenes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-26 |
| Detector | ADSC QUANTUM 270 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 161.800, 62.500, 91.600 |
| Unit cell angles | 90.00, 120.50, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.22615 |
| Rwork | 0.225 |
| R-free | 0.25085 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cb1 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.942 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.128 | 0.458 |
| Number of reflections | 39656 | |
| <I/σ(I)> | 6.2 | |
| Completeness [%] | 96.8 | 98.6 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 295 | 25-30% (w/v) ethylene glycol and 5% (v/v) N-dodecyl-N, N-dimethylamine-N-oxide, pH 8, vapor diffusion, hanging drop, temperature 295K |
