3RHG

Crystal structure of amidohydrolase pmi1525 (target efi-500319) from proteus mirabilis hi4320

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	90
Detector technology	CCD
Collection date	2009-03-17
Detector	ADSC QUANTUM 315
Spacegroup name	P 32 2 1
Unit cell lengths	101.203, 101.203, 65.614
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.530
R-factor	0.12095
Rwork	0.120
R-free	0.15637
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3htw
RMSD bond length	0.010
RMSD bond angle	1.227
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.000	1.560
High resolution limit [Å]	1.530	1.530
Rmerge		0.800
Number of reflections	58633
<I/σ(I)>	9.9	2.2
Completeness [%]	99.7	95
Redundancy	17.7	15.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		0.1M SODIUM CACODYLATE, PH 6.5, 0.2M AMMONIUM SULFATE, 30% PEG8000, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K