3RH9
The crystal structure of oxidoreductase from Marinobacter aquaeolei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.483, 102.477, 144.498 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.317 - 2.630 |
| R-factor | 0.1911 |
| Rwork | 0.187 |
| R-free | 0.25170 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.153 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.720 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Rmerge | 0.143 | 0.449 |
| Number of reflections | 30524 | |
| <I/σ(I)> | 35.9 | 5.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.5 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 293 | 0.1 M Citric acid pH 3.5, 25% w/v PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
