3RH6
DNA Polymerase Beta Mutant (Y271) with a dideoxy-terminated primer with an incoming ribonucleotide (rCTP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-24 |
| Detector | RIGAKU SATURN 92 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.060, 79.810, 55.060 |
| Unit cell angles | 90.00, 107.68, 90.00 |
Refinement procedure
| Resolution | 20.770 - 2.048 |
| R-factor | 0.2017 |
| Rwork | 0.195 |
| R-free | 0.26580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fms |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.182 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.770 | 2.120 |
| High resolution limit [Å] | 2.048 | 2.050 |
| Rmerge | 0.111 | 0.521 |
| Number of reflections | 25790 | |
| <I/σ(I)> | 11.3 | 2.28 |
| Completeness [%] | 97.2 | |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 16% PEG3350, 350 mM sodium acetate, 50 mM imidazole, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
