3RH0

Corynebacterium glutamicum mycothiol/mycoredoxin1-dependent arsenate reductase Cg_ArsC2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 1
Unit cell lengths	36.350, 38.760, 43.860
Unit cell angles	100.60, 102.54, 99.94

Refinement procedure

Resolution	31.575 - 1.724
R-factor	0.1796
Rwork	0.177
R-free	0.23690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1je3
RMSD bond length	0.006
RMSD bond angle	1.013
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.575	1.820
High resolution limit [Å]	1.724	1.724
Number of reflections	22115
Completeness [%]	93.5	93.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	20% PEG 10000, 0.1M Hepes pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K