3RGZ
Structural insight into brassinosteroid perception by BRI1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2011-01-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 170.410, 66.820, 114.341 |
| Unit cell angles | 90.00, 119.50, 90.00 |
Refinement procedure
| Resolution | 49.759 - 2.281 |
| R-factor | 0.1763 |
| Rwork | 0.174 |
| R-free | 0.21830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ogq 1o6v |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.130 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.280 |
| Rmerge | 0.056 |
| Number of reflections | 49788 |
| Completeness [%] | 97.2 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.2M Na2SO4, 20% (w/v) PEG 3350, 10mM Trimethylamine-HCl , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
