3RGX
Structural insight into brassinosteroid perception by BRI1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2010-08-09 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.391, 66.705, 119.051 |
| Unit cell angles | 90.00, 120.98, 90.00 |
Refinement procedure
| Resolution | 48.010 - 2.470 |
| R-factor | 0.2016 |
| Rwork | 0.199 |
| R-free | 0.24560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ogq 1o6v |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.064 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.470 |
| Rmerge | 0.078 |
| Number of reflections | 41987 |
| Completeness [%] | 99.5 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.2M Na2SO4,20% (w/v) polyethylene glycol (PEG) 3,350, 10mM Trimethylamine-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
