3RGU
Structure of Fap-NRa at pH 5.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.939 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 121.885, 121.885, 117.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.430 - 3.000 |
| R-factor | 0.22387 |
| Rwork | 0.223 |
| R-free | 0.24194 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2kub |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.462 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0104) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 37.430 | 37.430 | 3.160 |
| High resolution limit [Å] | 3.000 | 9.490 | 3.000 |
| Rmerge | 0.080 | 0.049 | 0.393 |
| Number of reflections | 17284 | ||
| <I/σ(I)> | 10.6 | 18.5 | 2.8 |
| Completeness [%] | 94.9 | 81.2 | 95.9 |
| Redundancy | 4.5 | 3.8 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.9 M Na/K phosphate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293 K |
