3RGI
Trans-acting transferase from Disorazole synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-27 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.430, 54.140, 121.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.630 - 1.510 |
| R-factor | 0.16314 |
| Rwork | 0.162 |
| R-free | 0.18175 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g1h |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.511 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.900 | 1.550 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Number of reflections | 44785 | |
| <I/σ(I)> | 26.7 | 3.4 |
| Completeness [%] | 97.7 | 86.9 |
| Redundancy | 9.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 30% PEG3350, 40 mM ammonium acetate, 0.1 M Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
