3RGC

The virulence factor PEB4 and the periplasmic protein Cj1289 are two structurally related SurA-like chaperones in the human pathogen Campylobacter jejuni

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9840, 0.9801, 0.9803, 0.9686
Spacegroup name	P 21 21 21
Unit cell lengths	49.590, 94.610, 124.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.032 - 2.300
R-factor	0.2264
Rwork	0.224
R-free	0.27780
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.090
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.9)
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.300	62.300	2.420
High resolution limit [Å]	2.300	7.270	2.300
Rmerge		0.035	0.633
Rmeas		0.041	0.732
Rpim		0.016	0.272
Total number of observations		6000	27851
Number of reflections	26871
<I/σ(I)>	14.3	36.9	3.2
Completeness [%]	100.0	98.8	100
Redundancy	7	6.3	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	290	0.1M CHES pH 8.0, 25%(w/v) PEG 8000, 5% (w/v) sucrose, VAPOR DIFFUSION, SITTING DROP, temperature 290K