3RGB
Crystal structure of particulate methane monooxygenase from Methylococcus capsulatus (Bath)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.377,1.280 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 264.140, 264.140, 150.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.460 - 2.800 |
| R-factor | 0.26997 |
| Rwork | 0.269 |
| R-free | 0.29606 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.621 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.460 | 2.873 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 112261 | |
| Completeness [%] | 91.2 | 45.47 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 8-12% PEG8000, 0.2 M zinc acetate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
