3RG5
Crystal Structure of Mouse tRNA(Sec)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.872, 96.055, 71.841 |
| Unit cell angles | 90.00, 105.06, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.2173 |
| Rwork | 0.215 |
| R-free | 0.25284 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | human tRNA(Sec) |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.749 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 42189 | |
| <I/σ(I)> | 19.7 | 1.2 |
| Completeness [%] | 97.9 | 90.1 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.2 | 293 | 0.1 M MES, pH 5.2, 10 mM magnesium acetate, 2.0 M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
