3RFZ
Crystal structure of the FimD usher bound to its cognate FimC:FimH substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2009-10-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.07230 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 126.190, 95.900, 144.570 |
| Unit cell angles | 90.00, 112.13, 90.00 |
Refinement procedure
| Resolution | 59.130 - 2.800 |
| R-factor | 0.22574 |
| Rwork | 0.223 |
| R-free | 0.28181 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.089 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.130 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.074 | |
| Number of reflections | 76954 | |
| <I/σ(I)> | 8 | |
| Completeness [%] | 97.3 | 98.4 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | ammonium sulfate, ammonium acetate, isopropanol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
