3RFW

The virulence factor PEB4 and the periplasmic protein Cj1289 are two structurally-related SurA-like chaperones in the human pathogen Campylobacter jejuni

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9780
Spacegroup name	C 1 2 1
Unit cell lengths	81.353, 91.595, 61.553
Unit cell angles	90.00, 102.27, 90.00

Refinement procedure

Resolution	39.750 - 2.200
R-factor	0.2362
Rwork	0.234
R-free	0.27630
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.110
Data scaling software	SCALA (3.3.9)
Phasing software	SOLVE (2.13)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.797	45.797	2.320
High resolution limit [Å]	2.199	6.960	2.200
Rmerge		0.051	0.443
Rmeas		0.055	0.464
Rpim		0.020	0.138
Total number of observations		5478	36924
Number of reflections	22275
<I/σ(I)>	20.3	36.8	5.7
Completeness [%]	99.5	85.4	100
Redundancy	11.3	8.8	11.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.5	280	0.1M succinate, 40% v/v Jeffamine, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 280K
1	VAPOR DIFFUSION, SITTING DROP	3.5	280	0.1M succinate, 40% v/v Jeffamine, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 280K