3RFN
Epitope backbone grafting by computational design for improved presentation of linear epitopes on scaffold proteins
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 93.2 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-12-03 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.800, 36.100, 46.300 |
| Unit cell angles | 90.00, 99.40, 90.00 |
Refinement procedure
| Resolution | 17.000 - 1.800 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.23700 |
| Structure solution method | MIR |
| Starting model (for MR) | 1wnu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 15316 | |
| <I/σ(I)> | 25 | 5.9 |
| Completeness [%] | 97.7 | 89 |
| Redundancy | 1.75 | 1.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 2.8 M NaCl, 0.1 M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
