3RFE
Crystal structure of glycoprotein GPIb ectodomain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 193 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.506, 34.764, 71.532 |
| Unit cell angles | 90.00, 92.15, 90.00 |
Refinement procedure
| Resolution | 54.300 - 1.245 |
| R-factor | 0.202 |
| Rwork | 0.201 |
| R-free | 0.22433 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.489 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MrBUMP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 54.300 |
| High resolution limit [Å] | 1.245 |
| Number of reflections | 78352 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 1.6M(NH4)2SO4, 0.4M LiCl, 0.1M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
