3RF9

X-ray structure of RlmN from Escherichia coli

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	113
Detector technology	CCD
Collection date	2010-11-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.98, 1.65, 1.72
Spacegroup name	C 2 2 21
Unit cell lengths	72.152, 80.406, 312.242
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.200
R-factor	0.2159
Rwork	0.213
R-free	0.26690
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.012
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.086	0.049	0.453
Number of reflections	43897
<I/σ(I)>	8.5
Completeness [%]	99.3	99.9	92.3
Redundancy	7.2	6.9	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	0.1 M HEPES, 10% PEG 6000, 5% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K