3RF9
X-ray structure of RlmN from Escherichia coli
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 113 |
Detector technology | CCD |
Collection date | 2010-11-11 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.98, 1.65, 1.72 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 72.152, 80.406, 312.242 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.200 |
R-factor | 0.2159 |
Rwork | 0.213 |
R-free | 0.26690 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.012 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 5.970 | 2.200 |
Rmerge | 0.086 | 0.049 | 0.453 |
Number of reflections | 43897 | ||
<I/σ(I)> | 8.5 | ||
Completeness [%] | 99.3 | 99.9 | 92.3 |
Redundancy | 7.2 | 6.9 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | 0.1 M HEPES, 10% PEG 6000, 5% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |