3RF6

Crystal structure of glycerol-3 phosphate bound HAD-like phosphatase from Saccharomyces cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	59.912, 71.652, 195.428
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.239 - 1.695
R-factor	0.1657
Rwork	0.164
R-free	0.19350
Structure solution method	3KC2
RMSD bond length	0.021
RMSD bond angle	1.533
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.070
Number of reflections	93836
<I/σ(I)>	30
Completeness [%]	99.8	100
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	294	200mM NA citrate, HEPES, 2mM MGCL2, PH 7.5, 30mM glycerol-3-phosphate, VAPOR DIFFUSION, SITTING DROP, temperature 294K