3RF2

Crystal Structure of 30S Ribosomal Protein S8 from Aquifex Aeolicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-23
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	96.235, 96.235, 37.614
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.432 - 2.160
R-factor	0.2275
Rwork	0.223
R-free	0.26740
Structure solution method	MAD, MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.987
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.2.4)
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.160	4.650	2.160
Rmerge	0.062	0.043	0.236
Number of reflections	9918
<I/σ(I)>	14.4
Completeness [%]	99.3	99.4	96.1
Redundancy	12.3	13.4	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.6	295	40% PEG 400, 0.1M sodium acetate, 0.2M lithium sulphate, pH 4.6, VAPOR DIFFUSION, temperature 295K
1	VAPOR DIFFUSION	4.6	295	40% PEG 400, 0.1M sodium acetate, 0.2M lithium sulphate, pH 4.6, VAPOR DIFFUSION, temperature 295K