3REZ
glycoprotein GPIb variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2010-06-18 |
| Detector | MARC MOSAIC3 |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 72.010, 72.010, 171.731 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.170 - 2.350 |
| R-factor | 0.2139 |
| Rwork | 0.211 |
| R-free | 0.25925 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.301 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 62.360 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 23135 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 292 | 0.1 M MMT buffer pH 5 (MMT buffer is a mixture of DL-malic acid, MES and Tris base in the molar ratios 1:2:2 DL-malic acid:MES:Tris base), 25% (w/v) PEG 1500, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
