3REX

Crystal structure of the archaeal asparagine synthetase A complexed with Adenosine monophosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Detector technology	CCD
Collection date	2006-06-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.87300
Spacegroup name	P 21 21 21
Unit cell lengths	58.380, 60.930, 155.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.818 - 1.800
R-factor	0.1613
Rwork	0.160
R-free	0.19640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nnh
RMSD bond length	0.007
RMSD bond angle	1.101
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.900
High resolution limit [Å]	1.800	1.800
Number of reflections	51859
Completeness [%]	98.9	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	100mM Tris pH7 0.2M NaCl 32%PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K