3RES
Crystal structure of E coli Hfq in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 111.260, 34.120, 200.743 |
| Unit cell angles | 90.00, 98.39, 90.00 |
Refinement procedure
| Resolution | 102.840 - 2.000 |
| R-factor | 0.20072 |
| Rwork | 0.199 |
| R-free | 0.23864 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.483 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 102.843 | 30.576 | 2.000 |
| High resolution limit [Å] | 1.900 | 6.010 | 1.900 |
| Rmerge | 0.074 | 0.054 | 0.205 |
| Rmeas | 0.061 | 0.227 | |
| Rpim | 0.027 | 0.095 | |
| Total number of observations | 9730 | 43499 | |
| Number of reflections | 58163 | ||
| <I/σ(I)> | 12 | 20 | 5.3 |
| Completeness [%] | 97.6 | 93.2 | 95.6 |
| Redundancy | 5.4 | 5.2 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 283 | 200mM NH4Ac, 100mM NaAc, 22% PEG 4000, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
