3RD6
Crystal structure of Mll3558 protein from Rhizobium loti. Northeast Structural Genomics Consortium target id MlR403
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-07 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.837, 84.071, 108.932 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.510 - 2.800 |
R-factor | 0.245 |
Rwork | 0.245 |
R-free | 0.31000 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.860 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.051 | |
Number of reflections | 35914 | |
Completeness [%] | 97.5 | 98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1M HEPES (pH 7.5), 20% PEG4000, Na Citrate, VAPOR DIFFUSION, SITTING DROP, temperature 277K |