3RD3
Structure of Pseudomonas aeruginosa transcriptional regulator PA2196
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-10 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.396, 45.589, 66.840 |
Unit cell angles | 90.00, 109.72, 90.00 |
Refinement procedure
Resolution | 14.980 - 2.400 |
R-factor | 0.2207 |
Rwork | 0.217 |
R-free | 0.29470 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.710 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 13358 | |
Completeness [%] | 98.3 | 94.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 20% PEG 3350, 0.2M Na formate, 13% DMSO, pH 7.2, temperature 298K, VAPOR DIFFUSION, HANGING DROP |