3RCE
Bacterial oligosaccharyltransferase PglB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2010-11-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.060, 116.100, 175.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.978 - 3.400 |
| R-factor | 0.2408 |
| Rwork | 0.238 |
| R-free | 0.27100 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.475 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.600 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 24350 | |
| Completeness [%] | 99.3 | 97.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 9.4 | 298 | 0.1M glycine, 50mM magnesium acetate, 6% Dimethyl sulfoxide (DMSO), 23-34% PEG400, pH 9.4, EVAPORATION, temperature 298K |
