3RBW
Crystal structure of Spire KIND domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2009-02-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.328, 66.265, 100.852 |
| Unit cell angles | 90.00, 103.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.200 |
| R-factor | 0.26325 |
| Rwork | 0.261 |
| R-free | 0.31501 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3r7g |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.332 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 41.000 |
| High resolution limit [Å] | 3.200 |
| Rmerge | 0.061 |
| Number of reflections | 14983 |
| <I/σ(I)> | 14 |
| Completeness [%] | 94.3 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | VAPOR DIFFUSION |
