3RBS

Crystal structure of the myomesin domains 10 and 11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.91508
Spacegroup name	P 21 21 21
Unit cell lengths	38.187, 74.693, 89.414
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.495 - 1.850
R-factor	0.1903
Rwork	0.189
R-free	0.23900
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.024
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: dev_530))

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	1.850
Rmerge	0.470
Number of reflections	22541
<I/σ(I)>	33.9
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	292	0.2 M NaNO3, 18% w/v polyethylene glycol 3350, 5% w/w ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K