3RB8

Structure of the phage tubulin PhuZ(SeMet)-GDP

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97201, 0.97969
Spacegroup name	P 21 21 21
Unit cell lengths	46.981, 76.062, 92.758
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.379 - 2.600
R-factor	0.1706
Rwork	0.163
R-free	0.23730
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.141
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall
Low resolution limit [Å]	46.379
High resolution limit [Å]	2.400
Number of reflections	23023
Completeness [%]	91.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	15% PEG 6000, 0.1M Hepes, 0.5M Ammonium Acetate, 0.05M MgCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K