3RAY

Crystal structure of Methyltransferase domain of human PR domain-containing protein 11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2010-10-27
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	31.154, 55.156, 106.283
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.130 - 1.730
R-factor	0.1959
Rwork	0.194
R-free	0.22750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3db5
RMSD bond length	0.010
RMSD bond angle	0.990
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASES
Refinement software	BUSTER (2.8.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.730	1.730
Rmerge	0.036	0.678
Number of reflections	19744
<I/σ(I)>	59.8	2.1
Completeness [%]	99.4	100
Redundancy	7.6	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		297	20%PEG 3350, 0.2M KCl , VAPOR DIFFUSION, SITTING DROP, temperature 297K