3RAU
Crystal structure of the HD-PTP Bro1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-09 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.523, 61.333, 80.582 |
| Unit cell angles | 85.87, 81.27, 87.99 |
Refinement procedure
| Resolution | 46.958 - 1.950 |
| R-factor | 0.1745 |
| Rwork | 0.172 |
| R-free | 0.21530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oew |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.935 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 5.290 | 1.950 |
| Number of reflections | 49890 | ||
| Completeness [%] | 97.2 | 99.7 | 89.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 0.1M MES pH5.5, 16% PEG3350, 0.3M Magnesium Acetate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
