3RAM
Crystal structure of HmrA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2009-12-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 132.730, 132.730, 336.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.520 - 2.700 |
| R-factor | 0.19103 |
| Rwork | 0.191 |
| R-free | 0.21768 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.172 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.520 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.091 |
| Number of reflections | 83170 |
| Completeness [%] | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 278 | 49% MPD, 100mM Tris, 0.1mM ATP, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
