3RAB

GPPNHP-BOUND RAB3A AT 2.0 A RESOLUTION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-12
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	88.900, 35.000, 58.600
Unit cell angles	90.00, 107.20, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.191 *
Rwork	0.191
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5p21
RMSD bond length	0.007
RMSD bond angle	24.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.063 *	0.197 *
Number of reflections	10595
<I/σ(I)>	17.1	6.3
Completeness [%]	99.5	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	4 *	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	Tris-HCl	5 (mM)
3	1	drop		0.5 (mM)
4	1	reservoir	PEG8000	14 (%)
5	1	reservoir	NaMES	50 (mM)
6	1	reservoir		200 (mM)