3RAA

Structural studies of AAV8 capsid transitions associated with endosomal trafficking

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	113
Detector technology	CCD
Collection date	2008-06-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.990
Spacegroup name	P 63 2 2
Unit cell lengths	256.400, 256.400, 447.430
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.840 - 3.200
Rwork	0.294
R-free	0.29730
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.310
High resolution limit [Å]	3.200	3.200
Number of reflections	126615
Completeness [%]	89.0	88.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	20 mM Tris pH 7.5, 4% PEG 4000, 1 M NaCl. Crystals were pre-soaked at pH 4 for 24 hours and transferred back to pH7.5 before data collection, VAPOR DIFFUSION, HANGING DROP, temperature 293K