3RA7

Bispecific digoxigenin binding antibodies for targeted payload delivery

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9795
Spacegroup name	P 42 21 2
Unit cell lengths	138.013, 138.013, 123.617
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.040 - 2.798
R-factor	0.2054
Rwork	0.204
R-free	0.23120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cfd
RMSD bond length	0.006
RMSD bond angle	1.292
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.970
High resolution limit [Å]	2.798	2.800
Number of reflections	30038
Completeness [%]	70.5	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	298	60%(v/v) 2-methyl-1,3-propandiol(MPD), 0.1M sodium acetate, 5mM CaCl2, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K