3RA5
Crystal structure of T. celer L30e E6A/R92A variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-11-18 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 |
| Unit cell lengths | 64.245, 64.245, 48.401 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.122 - 1.800 |
| R-factor | 0.1944 |
| Rwork | 0.190 |
| R-free | 0.23470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h7m |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.014 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.500 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.064 | 0.153 |
| Number of reflections | 20739 | |
| <I/σ(I)> | 11.4 | 4.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 2.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 25% PEG 20000, 0.1M Tris, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
